Recombinant Factor C (rFC) kev soj ntsuamyog ib txoj kev siv dav rau kev kuaj cov kab mob endotoxins, tseem hu ua lipopolysaccharides (LPS), Endotoxins yog ib feem ntawm cov kab mob sab nrauv ntawm cov kab mob Gram-negative uas tuaj yeem ua rau muaj zog teb rau cov tsiaj, suav nrog tib neeg.Qhov kev ntsuam xyuas rFC yog ua raws li kev siv cov tshuaj genetic engineered daim ntawv ntawm Factor C, ib qho enzyme uas ib txwm muaj nyob rau hauv cov ntshav horseshoe crab thiab koom nrog hauv txoj hauv kev los ntshav.Hauv kev tshuaj ntsuam rFC, qhov sib xyaw ua ke C yog siv los kuaj pom muaj cov tshuaj endotoxins los ntawm kev ntsuas Los ntawm kev ntsuas cov ntsiab lus ntawm cleaved substrates nyob rau hauv lub xub ntiag ntawm endotoxin.Piv nrog rau cov txheej txheem ntawm kev tshawb nrhiav endotoxin ib txwm muaj, xws li Limulus Amebocyte Lysate (LAL) kev soj ntsuam uas siv cov ntshav horseshoe crab, rFC assay raug suav hais tias yog cov qauv tsim dua thiab rov tsim dua tshiab, vim nws tsis tso siab rau kev siv cov tshuaj tua tsiaj.Qhov kev ntsuam xyuas rFC kuj yog ib puag ncig zoo thiab ruaj khov, vim nws txo qhov kev xav tau ntawm kev sau thiab siv horseshoe crabs hauv kev tshawb nrhiav endotoxin.
Qhov kev ntsuam xyuas rFC tau pom zoo los ntawm cov thawj coj tswj hwm, xws li Tebchaws Meskas Pharmacopeia (USP), European Pharmacopoeia (EP) thiab Suav Pharmacopoeia (CP) rau kev siv hauv kev tswj xyuas cov tshuaj thiab cov khoom siv kho mob zoo.
Qhov zoo ntawm recombinant factor c assay
Qhov kev ntsuam xyuas Recombinant Factor C (rFC) muaj ntau qhov zoo dua li cov txheej txheem ib txwm siv los kuaj xyuas cov tshuaj endotoxins, xws li Limulus Amebocyte Lysate (LAL) kev soj ntsuam.Qee qhov zoo ntawm qhov kev ntsuam xyuas rFC suav nrog:
1. Standardization: rFC assay yog ib tug recombinant DNA technology uas siv ib zaug xwb, txhais tau tias protein raws li lub nrhiav kom tau reagent.Qhov no ua rau qhov kev ntsuam xyuas ntau dua tus qauv thiab tsis tshua muaj qhov sib txawv piv rau LAL kev soj ntsuam, uas tso siab rau kev siv cov sib xyaw ua ke ntawm cov protein uas tau muab rho tawm los ntawm cov ntshav horseshoe crab.
2. Reproducibility: Qhov kev ntsuam xyuas rFC muaj qib siab ntawm kev rov ua dua tshiab, raws li nws siv ib leeg, txhais cov protein raws li kev tshawb nrhiav reagent.Qhov no tso cai rau cov txiaj ntsig zoo ib yam, txawm tias hla cov khoom sib txawv thiab ntau cov tshuaj reagents.
3. Kev siv tsiaj tsawg: Kev ntsuam xyuas rFC yog ib txoj hauv kev zoo tshaj plaws thiab ruaj khov rau kev kuaj xyuas endotoxins, vim nws tsis tas yuav tsum siv cov tsiaj nyob lossis txi txiv, xws li horseshoe crabs.
4. Tus nqi tsim nyog: Kev ntsuam xyuas rFC feem ntau yog tus nqi ntau dua li qhov kev ntsuam xyuas LAL, vim tias qhov kev xav tau ntawm cov tsiaj nyob qis dua thiab cov qauv kev ntsuas ntau dua.
5. Kev ruaj ntseg: Qhov kev ntsuam xyuas rFC yog qhov muaj zog thiab tuaj yeem siv ntau yam kev siv, suav nrog kev tswj xyuas zoo ntawm cov tshuaj, cov khoom siv kho mob, thiab lwm yam khoom uas yuav muaj cov tshuaj endotoxins.
6. Kev pom zoo raws li txoj cai: rFC qhov kev soj ntsuam tau raug pom zoo los ntawm cov cai tswj hwm xws li Tebchaws Meskas Pharmacopeia (USP), European Pharmacopoeia (EP) thiab Suav Pharmacopoeia (CP) rau kev siv hauv kev tswj xyuas qhov zoo ntawm cov tshuaj thiab cov khoom siv kho mob.Qhov no ua rau muaj kev ntseeg siab hauv kev ntseeg siab thiab qhov tseeb ntawm qhov kev ntsuam xyuas.
Txhawm rau kom tau raws li qhov xav tau ntau yam, Bioendo tseem tsim thiab muab cov txheej txheem ib txwm siv ntawm gel clot endotoxin test assay kit, ceev gel clot assay kit, quantitative endotoxin test assay kit nrog rau "kinetic turbidimetric endotoxin test assay kitthiabKinetic chromogenic endotoxin kuaj cov khoom siv”.
Post lub sij hawm: Feb-19-2023
