Endotoxin Test Assay los ntawm Lyophilized Amebocyte Lysate (LAL Reagent)
LAL Reagents: Lyophilized amebocyte lysate (LAL) yog ib qho aqueous extract ntawm cov ntshav hlwb (amebocytes) los ntawm Atlantic horseshoe crab.
TAL Reagents: TAL reagent yog ib qho aqueous extract ntawm cov qe ntshav los ntawm Tachypleus tridentatus.
Tam sim no, cov khoom tseem ceeb ntawm LAL / TAL reagents yog nyob rau hauv Tebchaws Meskas thiab Tuam Tshoj.
Gel clot endotoxin test assay, txoj kev no tseem yog cov ntawv thov tseem ceeb hauv thoob plaws ntiaj teb, txhawm rau ua kom tau raws li qhov xav tau kev nyab xeeb ntawm Tib neeg cov tshuaj txhaj tshuaj.
Tam sim no Bioendo tsim thiab muab cov gel clot LAL reagent suav nrog rau ib qho kev sim iav ampules thiab ntau lub vials.
https://www.bioendo.com/gel-clot-endotoxin-assay/ G01, GS44, G02, G17 thiab G52
Qhov no yog qhov kev daws teeb meem kev lag luam rau kev tshawb nrhiav endotoxins zoo hauv kev sim tshuaj.Tshwj xeeb tshaj yog rau cov tshuaj txhaj tshuaj lossis cov tshuaj parenteral los kuaj endotoxins hauv WFI, API lossis cov khoom siv tshuaj tiav.Endotoxin test assay yog qhov kev thov siab ntawm kev ua haujlwm, xav tau tus neeg ua haujlwm paub zoo los cuam tshuam nrog gel clot assay kom paub meej qhov tseeb.
Ib qho kev daws teeb meem tiav rauG52Kev ntsuam xyuas Endotoxin:
LAL Reagent
Tswj Standard Endotoxin
BET Dej
Endotoxin-dawb pipette tswv yim
Endotoxin-dawb iav raj, nrog rau kev ua haujlwm dilution thiab cov raj tshuaj tiv thaiv.
Incubation ntsuas, kom pom zoo da dej los yog qhuav cua sov incubator.Tag nrho cov cuab yeej incubation yuav tsum tau kub precision.
Txhua yam khoom siv los kov LAL kev soj ntsuam uas ua tau raws li tus qauv ntawm endotoxin-dawb qib ” < 0.005EU / ml ” .
Kev sim ib puag ncig yuav tsum haum rau kev tshawb nrhiav endotoxin.
Them rau:
Cov khoom siv yas zoo li cov lus qhia pipette lossis daim hlau multiwell tau dav siv rau kev kuaj endotoxin.Lub harmonized Pharmacopoeias (USP / CP) xav kom txhua yam khoom siv yas thiab cov iav raj yuav tsum tsis muaj cov tshuaj endotoxin thiab tseem ceeb sib npaug tab sis feem ntau tsis xav txog, lawv yuav tsum muaj tus qauv tsis muaj kev cuam tshuam.
Yuav ua li cas khiav qhov kev ntsuam xyuas endotoxin los kuaj xyuas cov endotoxins hauv cov qauv?
Ua ntej ntawm tag nrho cov, kev ntsuam xyuas rau kev pom zoo ntawm daim ntawv lo lysate rhiab heev yuav tsum tau nqa tawm.Kom paub meej tias qhov rhiab heev yog tib yam rau cov ntawv cim.
Rau cov qauv kev soj ntsuam, ua qhov kev ntsuam xyuas ua ntej cuam tshuam endotoxin.
Txhawm rau ua tiav qhov kev ntsuam xyuas endotoxin ntawm "kev cuam tshuam kev sim".
Txhawm rau ua haujlwm qhov kev txwv txwv rau qhov tau txais yuav ua li cas qib endotoxins hauv cov qauv.
Thaum ua haujlwm qhov kev ntsuam xyuas rau kev pom zoo ntawm daim ntawv lo lysate rhiab heev, qhov tshwm sim yog txawv txav, 2 Lamada tsis muaj gel tsim?
Xyuas seb txoj kev npaj ntawm cov kab mob tswj cov txheej txheem endotoxin puas yog.
Kev sib xyaw vortex yuav tsum muaj rau txhua qhov diluent (saib phau ntawv qhia lossis hu ua ntxig ntawm cov txheej txheem tswj endotoxin hauv cov ntsiab lus).
Txhawm rau xyuas seb cov lysate reagent puas haum rau tus qauv tswj endotoxin.
Nws raug nquahu kom siv cov dej da dej lossis lub tshuab ziab khaub ncaws qhuav rau cov txheej txheem kub tsis tu ncua ntawm lysate reagent (piv txwv li, lub tshuab nqus tsev lossis lub thawv qhuav tsis zoo).
Thaum lub sij hawm incubation, dej ib puag ncig hauv cov dej da dej uas zoo li qub thiab ruaj khov, tag nrho cov kev vibrations raug zam.
Ua tib zoo saib xyuas cov dej da dej kom kaw lub twj tso kua mis dej ntws.
Kev txiav txim siab qhov tshwm sim, maj mam tshem lub raj los yog iav ampoule los ntawm lub ntsuas sov, maj mam tig 180 degrees,
gel tsim nyob rau hauv lub raj yuav tsis deformed, thiab yuav tsis plam tawm, qhov tshwm sim tau kaw los ntawm lub teeb liab "+";
tsis muaj gel tsim los yog cov gel clot tsis tuaj yeem khaws cia zoo txawm tias tsim.
Lub phab ntsa plam yog qhov tsis zoo, qhov tshwm sim tau kaw los ntawm lub teeb liab "-".
Rapid gel clot endotoxin test Kit belongs rau gel clot endotoxin test method.
Hauv kev sim ceev gel clot endotoxin, cov qauv zoo tswj tsis tsim cov gel?
Ua ntej, siv cov kab mob endotoxin kuaj dej los xyuas cov lysate reagents, xyuas kom cov neeg ua haujlwm thiab ib puag ncig tau ua raws li cov cai;
Yog tias cov khoom tsim nyog, ces qhov kev tswj xyuas zoo ntawm cov qauv tsis gel tsim vim qhov cuam tshuam inhibitory ntawm cov qauv, thiab cov qauv yuav tsum tau ua tiav thiab kho.
Feem ntau cov qauv ua qauv yog dilution.
Post lub sij hawm: Dec-29-2021
