Limulus amebocyte lysate (LAL) los yog Tachypleus tridentatus lysate (TAL) yog ib qho aqueous extract ntawm cov qe ntshav los ntawm horseshoe crab.
Thiab endotoxins yog hydrophobic molecules uas yog ib feem ntawm lipopolysaccharide complex uas tsim feem ntau ntawm cov txheej txheem ntawm cov kab mob Gram-negative.Cov khoom parenteral kis nrog pyrogens tuaj yeem ua rau muaj qhov tshwm sim loj xws li kub taub hau, poob siab, lub cev tsis ua haujlwm, lossis tuag taus.
LAL / TAL reagent tuaj yeem cuam tshuam nrog cov kab mob endotoxin thiab lipopolysaccharide (LPS).LAL's endotoxin binding thiab clotting peev xwm yog dab tsi ua rau nws muaj nuj nqis rau peb tus kheej kev lag luam kws tshuaj.Thiab qhov no yog vim li cas LAL / TAL reagent tuaj yeem ua haujlwm los kuaj lossis ntsuas cov kab mob endotoxin.
Ua ntej qhov kev tshawb pom tias LAL / TAL tuaj yeem siv los kuaj cov kab mob endotoxins, cov luav tau ua haujlwm los kuaj xyuas thiab ntsuas cov tshuaj endotoxins hauv cov khoom siv tshuaj.Piv nrog RPT, BET nrog LAL / TAL reagent yog nrawm thiab ua haujlwm tau zoo, thiab nws yog txoj hauv kev nrov los saib xyuas cov tshuaj endotoxin concentration hauv kev lag luam tshuaj, thiab lwm yam.
Lub gel clot endotoxin test assay, tseem hu ua Limulus Amebocyte Lysate (LAL) test, los yog hu ua Lyophilized Amebocyte Lysate (LAL) yog ib txoj hauv kev siv los kuaj thiab ntsuas cov tshuaj endotoxins hauv ntau yam khoom, tshwj xeeb hauv kev lag luam tshuaj thiab kho mob.Nws yog suav tias yog ib qho kev daws teeb meem tsim nyog hauv kev tshawb nrhiav endotoxin vim nws cov txiaj ntsig zoo thiab kev lees paub.
Kev kuaj LAL yog ua raws li lub hauv paus ntsiab lus uas cov qe ntshav ntawm horseshoe crabs (Limulus polyphemus los yog Tachypleus tridentatus) muaj cov kab mob uas ua rau muaj cov kab mob endotoxins, uas ua rau tsim cov gel zoo li cov hlab ntsha.Cov tshuaj tiv thaiv no yog cov rhiab heev thiab tshwj xeeb rau endotoxins, uas yog cov tshuaj lom ntawm cov kab mob gram-tsis zoo.
Muaj ntau ntau yam laj thawj vim li cas gel clot endotoxin test assay yog suav tias yog ib qho kev daws teeb meem tsim nyog hauv kev tshawb nrhiav endotoxin:
1. Regulatory Txais: Qhov kev xeem LAL tau lees paub thiab lees paub los ntawm cov cai tswj hwm xws li Tebchaws Meskas Pharmacopeia (USP) thiab European Pharmacopoeia (EP) raws li tus qauv qauv rau kev kuaj endotoxin.Ua raws li cov cai no yuav tsum tau ua kom muaj kev nyab xeeb thiab zoo ntawm cov khoom siv tshuaj.
2. rhiab heev thiab Specificity: LAL xeem muaj ib tug siab rhiab heev, tso cai rau kom paub txog cov endotoxins tsawg heev.Nws muaj peev xwm txheeb xyuas cov endotoxin concentrations tsawg li 0.01 endotoxin units ib milliliter (EU/mL).Qhov tshwj xeeb ntawm qhov kev sim ua kom ntseeg tau tias nws feem ntau kuaj pom cov tshuaj endotoxins thiab txo qis cov txiaj ntsig tsis tseeb.
3. Tus Nqi-Kev Ua Tau Zoo: Cov gel clot endotoxin test assay feem ntau suav hais tias yog ib qho kev daws teeb meem nyiaj txiag piv rau lwm txoj hauv kev xws li chromogenic lossis turbidimetric assay.Nws yuav tsum muaj tsawg dua reagents thiab khoom siv, txo cov nqi kuaj tag nrho.Tsis tas li ntawd, qhov muaj cov qauv LAL reagents nyob rau hauv kev ua lag luam ua rau nws yooj yim rau lub chaw soj nstuam los ua qhov kev xeem.
4. Kev Lag Luam Txuj Ci: LAL xeem tau dav siv hauv kev lag luam kws tshuaj thiab khoom siv kho mob raws li tus qauv txheej txheem rau kev kuaj pom endotoxin.Nws yog ib feem tseem ceeb ntawm cov txheej txheem tswj kev ua tau zoo thaum lub sij hawm tsim cov khoom siv tshuaj thiab cov khoom siv kho mob, ua kom muaj kev ua raws li kev cai tswj hwm.
Txawm li cas los xij, nws tsim nyog sau cia tias qhov gel clot endotoxin test assay tej zaum yuav muaj kev txwv, xws li cuam tshuam los ntawm qee yam tshuaj thiab muaj peev xwm ua rau qhov tsis zoo lossis tsis tseeb-tsis zoo.Hauv cov xwm txheej tshwj xeeb, lwm txoj hauv kev xws li chromogenic lossis turbidimetric kev soj ntsuam yuav raug siv los ntxiv lossis siv tau cov txiaj ntsig tau los ntawm LAL xeem.
Post lub sij hawm: Apr-29-2019
