KCET- Kinetic Chromogenic Endotoxin Test Assay (Chromogenic endotoxin test assay yog txoj hauv kev tseem ceeb rau cov qauv nrog qee qhov cuam tshuam.)
Kev ntsuam xyuas kinetic chromogenic endotoxin test (KCT lossis KCET) yog ib txoj hauv kev siv los kuaj pom muaj cov endotoxins hauv cov qauv.
Endotoxins yog cov tshuaj lom neeg muaj nyob hauv cov phab ntsa ntawm tes ntawm qee hom kab mob, suav nrog cov kab mob gram-tsis zoo xws li Escherichia coli thiab Salmonella.Hauv KCET qhov kev ntsuam xyuas, ib qho chromogenic substrate tau ntxiv rau cov qauv, uas cuam tshuam nrog cov endotoxins tam sim no los tsim cov xim hloov.
Tus nqi ntawm kev loj hlob xim yog saib xyuas lub sij hawm siv lub spectrophotometer, thiab tus nqi ntawm endotoxin nyob rau hauv cov qauv yog xam raws li tus nqi no.
KCT assay yog ib txoj hauv kev zoo los kuaj xyuas cov tshuaj endotoxins hauv cov chaw muag tshuaj, cov khoom siv kho mob, thiab lwm yam khoom uas tuaj yeem cuam tshuam nrog tib neeg lub cev.Nws yog ib qho kev ntsuam xyuas rhiab thiab txhim khu kev qha uas tuaj yeem tshawb pom txawm tias tsawg heev ntawm endotoxin, ua rau nws yog ib qho tseem ceeb rau kev ruaj ntseg ntawm cov khoom no.
TAL / LAL reagent yog lyophilized amebocyte lysate uas tau muab rho tawm los ntawm cov ntshav xiav ntawm Limulus polyphemus lossis Tachypleus tridentatus.
Endotoxins yog amphiphilic lipopolysaccharides (LPS) nyob rau hauv lub cell membrane ntawm cov kab mob gram-tsis zoo.Cov khoom lag luam parenteral kis nrog pyrogens suav nrog LPS tuaj yeem ua rau muaj kev txhim kho kub taub hau, ua rau cov lus teb inflammatory, poob siab, lub cev tsis ua haujlwm thiab kev tuag ntawm tib neeg.
Yog li ntawd, cov teb chaws thoob plaws ntiaj teb tau tsim cov kev cai, xav kom cov khoom lag luam uas thov kom tsis muaj menyuam thiab tsis yog pyrogenic yuav tsum tau sim ua ntej tso tawm.Gel-clot TAL assay yog thawj zaug tsim rau cov kab mob endotoxins kuaj (piv txwv li BET).
Txawm li cas los xij, lwm txoj hauv kev siab dua ntawm TAL kev soj ntsuam tau tshwm sim.Thiab cov txheej txheem no yuav tsis tsuas yog kuaj pom tab sis kuj suav tias muaj cov endotoxins hauv cov qauv.Sib nrug los ntawm cov txheej txheem gel-clot, cov txheej txheem rau BET kuj muaj cov txheej txheem turbidimetric thiab cov txheej txheem chromogenic.Bioendo, mob siab rau kev tshawb nrhiav endotoxin, yog cov kws tshaj lij tsim khoom los tsim cov tshuaj chromogenic TAL / LAL.
Bioendo EC Endotoxin Test Kit (End-point Chromogenic Assay) muab kev ntsuas ceev rau endotoxin kom muaj nuj nqis.
Peb kuj muab Bioendo KC Endotoxin Test Kit (Kinetic Chromogenic Assay) thiab incubation microplate nyeem ELx808IU-SN, uas tuaj yeem ua kom ntseeg tau thiab ua haujlwm ntawm koj qhov kev sim.
Dab tsi yog cov yam ntxwv ntawm lubkinetic chromogenic endotoxin test assaykuaj endotoxins hauv cov qauv?
Lub kinetic chromogenic endotoxin test assay yog lwm txoj hauv kev siv los kuaj cov tshuaj endotoxins hauv cov qauv.Nws muaj ntau yam nta:
1. Kinetic ntsuas: Zoo ib yam li qhov ntsuas turbidimetric, qhov kev ntsuas kinetic chromogenic kuj suav nrog kev ntsuas kinetic.Nws tso siab rau cov tshuaj tiv thaiv ntawm endotoxins thiab chromogenic substrate los tsim cov khoom xim.Cov kev hloov ntawm cov xim siv lub sij hawm yog saib xyuas, tso cai rau kom muaj nuj nqis ntawm endotoxin concentrations hauv cov qauv.
2. Siab rhiab heev: kinetic chromogenic assay yog rhiab heev thiab tuaj yeem kuaj xyuas cov endotoxins tsawg hauv cov qauv.Nws tuaj yeem ntsuas qhov ntsuas endotoxin kom raug, txawm tias nyob rau theem qis heev, ua kom ntseeg tau tias muaj kev ntseeg tau thiab ntsuas kom muaj nuj nqis.
3. Dav dynamic ntau yam: Qhov kev ntsuam xyuas muaj qhov dav dynamic ntau yam, tso cai rau kev ntsuas ntawm endotoxin concentrations thoob plaws ib qho dav spectrum.Qhov no txhais tau hais tias nws tuaj yeem kuaj cov qauv nrog ntau qib ntawm endotoxins, ua kom haum ob qho tib si qis thiab siab ntau yam tsis tas yuav muaj cov qauv dilution lossis concentration.
4. Cov txiaj ntsig tau nrawm: Kev ntsuas kinetic chromogenic muab cov txiaj ntsig sai piv rau cov txheej txheem ib txwm muaj.Nws feem ntau muaj lub sijhawm soj ntsuam luv dua, ua kom muaj kev sim sai thiab kev tshuaj xyuas cov qauv.Kev txhim kho xim tuaj yeem raug saib xyuas hauv lub sijhawm tiag tiag, thiab cov txiaj ntsig tau feem ntau tuaj yeem tau txais nyob rau hauv ob peb feeb mus rau ob peb teev, nyob ntawm seb cov khoom siv ntsuas tshwj xeeb thiab cov cuab yeej siv.
5. Automation thiab standardization: Qhov kev ntsuam xyuas tuaj yeem ua tau los ntawm kev siv tshuab, xws li microplate nyeem lossis
endotoxin tshwj xeeb analyzers.Qhov no tso cai rau kev ntsuas siab dhau los thiab ua kom muaj kev ntsuas zoo ib yam thiab cov qauv ntsuas, txo tib neeg kev ua yuam kev thiab nce kev ua haujlwm.
6. Kev sib raug zoo nrog ntau hom qauv: Kev ntsuas kinetic chromogenic yog sib xws nrog ntau hom qauv, suav nrog cov tshuaj, khoom siv kho mob, biologics, thiab cov qauv dej.Nws yog ntau txoj hauv kev uas tuaj yeem siv rau kev lag luam sib txawv thiab cov ntawv thov uas yuav tsum tau kuaj endotoxin.
Zuag qhia tag nrho, kinetic chromogenic endotoxin test assay muaj cov txheej txheem rhiab, nrawm, thiab txhim khu kev qha rau kev kuaj xyuas thiab ntsuas.
endotoxins hauv cov qauv.Nws yog dav siv hauv kev lag luam tshuaj, biotechnology, thiab kev kho mob kev lag luam rau kev tswj kom zoo thiab kev nyab xeeb
kev soj ntsuam lub hom phiaj.
Post lub sij hawm: Jul-29-2019
