Kev kuaj kab mob endotoxin (BET) tau ua nyob rau hauv cov chaw kuaj mob niaj hnub no nyob rau hauv kev tswj xyuas raws li qhov tseem ceeb kom tsis txhob cuam tshuam.
Tsim nyogtxheej txheem asepticyog ib qho tseem ceeb thaum npaj thiab diluting cov qauv thiab tuav cov qauv.Hnav khaub ncawsxyaum sab nraum lub chaw kuaj mob ib txwm muaj cov cuab yeej tiv thaiv tus kheej (PPE) cov kev cai yuav tsis muaj kev txhawj xeeb tshwj tsis yog tias cov khoom nyob rau hauv kev sim xav tau cov kws tshuaj ntsuam xyuas kev nyab xeeb tshwj xeeb vim muaj tshuaj lom lossis kis kab mob.Hnab looj tesyuav tsum yog TALC-dawb, vim TALC yuav muaj cov theem tseem ceeb ntawm endotoxins.Phaj nyeem ntawv, da dej da dej, thiab qhuav cua sov thaivsiv rau cov qauv incubation yuav tsum nyob rau ntawm lub rooj ntev zaum sim kom deb ntawm cua sov, qhov cua, thiab cua txias (HVAC) ducts, muaj kev vibration tseem ceeb, thiab lub chaw soj nstuam tsheb uas yuav cuam tshuam rau qhov kev xeem.Qauv tuav lub sij hawm thiab tej yam kev mobyuav tsum tau txiav txim siab thiab muab cov ntaub ntawv tom qab, yog tias tsim nyog, kom ntseeg tau tias cov txiaj ntsig kev xeem raug raug tsim tawm hauv lub sijhawm tsim nyog.
Piv txwv li, yog tias chav kuaj tau txais Cov Dej Txhaum Txhaum Cai (WFI) lossis cov qauv hauv kev ua haujlwm, nws puas yuav tsum tau tso rau hauv tub yees lossis nws puas tuaj yeem nyob hauv chav sov, thiab ntev npaum li cas?Ua ntej kev sim, nws raug nquahu kom cov qauv coj mus kuaj kom txaus sib xyaw ua ntej tshem tawm qhov kev xeem aliquot(s) rau kev kuaj ncaj qha lossis tom qab dilution.
Bioendo Bacterial Endotoxin Test, cov kev sim no suav nroggel clot methodendotoxin test assay thiabquantitative endotoxin test assay, gel clot method endotoxin test assay yog qhov zoo tshaj plaws endotoxin nrhiav pom, cov kev sim no xav tau cov khoom siv yog depyrogenation ua, xws li endotoxin dawb cov tshuaj tiv thaiv hlab, dilution hlab thiab cov lus qhia dawb pyrogen;Kev tshawb nrhiav ntau endotoxin muaj kinetic chromogenic endotoxin test, kinetic turbidimetric endotoxin test, cov kev sim no xav tau cov khoom siv yuav tsum tau ua kom tau raws li qib siab ntawm endotoxins tsawg dua.0.005EU/ml(0.001EU/ml), xws li endotoxin dawb hlab, pyrogen dawb cov lus qhia, thiab pyrogen dawb microplates, txawm pyrogen dawb resevoir.Los ntawm txoj kev, yog tias cov qauv kev kho mob, lub thawv yuav tsum yog endotoxin dawb qauv lub raj mis.
Hauv kev sim tshuaj endotoxin, kev cuam tshuam tuaj yeem tshwm sim los ntawm ntau qhov chaw, xws li cov qauv matrix, cov tshuaj ntsuam xyuas, lossis cov khoom siv.
Txhawm rau zam kev cuam tshuam kev sim, cov kev ntsuas hauv qab no tuaj yeem ua:
1. Kev Npaj Ua Qauv: Kev npaj kom zoo yog qhov tseem ceeb rau kev kuaj endotoxin kom raug.
Cov qauv matrix yuav tsum tau kuaj kom zoo thiab ua kom zoo kom ntseeg tau tias muaj kev sib raug zoo nrog cov tshuaj endotoxin.
Tshwj xeeb, cov khoom cuam tshuam xws li lipids thiab cov protein yuav tsum raug tshem tawm lossis txo qis siv cov txheej txheem tsim nyog xws li pom lossis centrifugation.
2. Kev tswj qhov zoo thiab tsis zoo: Nws yog ib qho tseem ceeb uas yuav tsum muaj kev tswj xyuas qhov zoo thiab qhov tsis zoo hauv qhov kev ntsuam xyuas los saib xyuas kev cuam tshuam.
Kev tswj tau zoo lees paub qhov ua haujlwm ntawm qhov kev sim, thaum cov kev tswj tsis zoo pom muaj kev sib kis lossis cuam tshuam los ntawm cov khoom siv tshuaj ntsuam xyuas.
3. Kev Tswj Xyuas Zoo: Kev tswj xyuas zoo yuav tsum tau ua ntawm txhua qhov reagents, khoom siv, thiab dej siv hauv kev ntsuam xyuas.
Qhov no ua kom ntseeg tau tias cov tshuaj reagents yog dawb los ntawm cov kab mob endotoxin thiab ua haujlwm kom raug.
4. Standardization: Qhov kev ntsuam xyuas yuav tsum tau ua qauv los xyuas kom meej tias tag nrho cov txiaj ntsig sib piv thiab rov tsim dua.
Qhov no suav nrog kev siv tus qauv nkhaus los ntsuas qhov ntsuas thiab siv cov txheej txheem txheej txheem rau kev npaj ua qauv, tsim, thiab tshawb pom.
5. Kev lees paub tseeb: Qhov kev ntsuam xyuas yuav tsum tau ua kom ntseeg tau tias nws yog qhov tshwj xeeb, nkag siab, thiab ntseeg tau.
Qhov no suav nrog kev sim ntau yam qauv, suav nrog cov paub tias muaj endotoxin, txhawm rau txiav txim siab qhov tseeb thiab qhov tseeb ntawm qhov ntsuas.
Los ntawm kev ua raws li cov kev ntsuas no, kev cuam tshuam tuaj yeem txo qis, thiab kev kuaj endotoxin muaj tseeb tuaj yeem ua tiav.
Post lub sij hawm: Dec-01-2022
