Cov iav raj nrog kev ua depyrogenation yog tsim nyog nyob rau hauv qhov kev ntsuam xyuas endotoxin los xyuas kom meej qhov tseeb thiab kev ntseeg siab ntawm cov txiaj ntsig kev xeem.Endotoxins yog cov khoom siv hluav taws xob ruaj khov ntawm cov phab ntsa ntawm tes sab nraud ntawm qee cov kab mob gram-tsis zoo, thiab lawv tuaj yeem ua rau mob hnyav thiab txawm tias tuag rau tib neeg yog tias lawv muaj nyob hauv cov khoom siv kho mob lossis khoom siv.
Txhawm rau txheeb xyuas cov tshuaj endotoxins, qhov kev ntsuam xyuas siv cov tshuaj reagents uas muaj Limulus Amebocyte Lysate (LAL) los yog hu ua Lyophilized amebocyte lysate, ib qho extract los ntawm horseshoe crab cov qe ntshav uas muaj cov txheej txheem ua kom lub cev los ntawm endotoxins.Txawm li cas los xij, cov iav raj uas tsis tau depyrogenated tuaj yeem cuam tshuam nrog LAL test assat los ntawm kev ua kom nws cov txheej txheem khov thiab ua rau cov txiaj ntsig tsis zoo.Yog li ntawd, cov iav raj siv nyob rau hauv qhov kev ntsuam xyuas endotoxin yuav tsum tau depyrogenated kom tshem tawm cov endotoxins uas yuav muaj nyob thiab tiv thaiv kom tsis txhob ua kom LAL reagent.Qhov no ua kom ntseeg tau tias cov txiaj ntsig ntawm kev kuaj endotoxin yog qhov tseeb thiab txhim khu kev qha thiab cov neeg mob tsis raug cuam tshuam rau qib siab ntawm endotoxins.thiab xyuas kom meej cov tshuaj parenteral kev nyab xeeb nyob rau hauv cov tshuaj, protains, cell kab lis kev cai, DNA thiab hais txog.
Qhov tsim nyog ntawm endotoxin-dawb iav raj siv nyob rau hauv endotoxin kuaj xyuas kev ua haujlwm:
Endotoxin-dawb iav rajyog ib qho tseem ceeb ntawm txhua qhov kev ntsuam xyuas endotoxin.Cov iav raj no yog tsim los txo qhov kev pheej hmoo ntawm endotoxin paug thaum lub sijhawm kuaj, ua kom cov txiaj ntsig raug thiab txhim khu kev qha.
Ib qho ntawm cov yam ntxwv tseem ceeb ntawm cov iav tsis muaj endotoxin yog lawv cov tshuaj muaj pes tsawg leeg.Cov raj no yog tsim los ntawm cov iav borosilicate zoo, uas paub txog nws qhov tshwj xeeb tsis kam tiv thaiv tshuaj corrosion.Qhov no ua rau lawv zoo tagnrho rau kev siv hauv kev kuaj endotoxin, vim tias lawv tuaj yeem tiv taus ntau yam ntawm cov tshuaj ntsuam xyuas yam tsis muaj degrading lossis kis tus qauv.
Lwm qhov tseem ceeb ntawm cov iav tsis muaj endotoxin yog lawv qhov huv.Cov raj no tau ua tib zoo ntxuav thiab ua kom tsis muaj menyuam ua ntej siv los tshem tawm tej qhov chaw muaj kab mob.Lawv kuj raug kuaj nruj me ntsis rau cov kab mob endotoxin, kom ntseeg tau tias lawv tsis muaj kab mob ntawm cov khoom tsis zoo no.
Tsis tas li ntawd, endotoxin-dawb iav raj yog tsim los siv tau yooj yim.Lawv feem ntau muaj nyob rau hauv ntau qhov ntau thiab tsawg thiab kev teeb tsa kom haum raws li cov qauv sib txawv thiab cov qauv kev sim ob qho tib si zoo endotoxin test assay thiab quantitative endotoxin test assay.Lawv kuj tau sib xws nrog ntau yam qauv kev npaj thiab kuaj cov cuab yeej, ua rau lawv muaj ntau yam thiab yooj yim xaiv rau cov chaw soj ntsuam endotoxin.
Zuag qhia tag nrho, endotoxin-dawb iav raj ua lub luag haujlwm tseem ceeb hauv qhov tseeb thiab kev ntseeg siab ntawm kev kuaj endotoxin.Lawv cov kev tsim kho zoo, purity, thiab yooj yim ntawm kev siv ua rau lawv yog ib qho tseem ceeb ntawm cov kev xeem endotoxin xeem.
Bioendo Endotoxin-dawb iav raj nrog loj10 * 75mm, 12 * 75mm, 13 * 100mm thiab 16 * 100mmrau cov txheej txheem dilutions thiab cov txheej txheem tshuaj tiv thaiv.
Endotoxin-dawb iav raj yog ua tau raws li cov txheej txheem saum toj kawg nkaus ntawm endotoxins tsawg dua 0.005EU / ml.
Endotoxin-dawb iav raj yuav tsum tau siv nyob rau hauv lub gel clot endotoxin test assay los tiv thaiv cov txiaj ntsig tsis tseeb.
Endotoxins yog cov kab mob ntawm cov phab ntsa ntawm cov phab ntsa uas tuaj yeem ua rau cov khoom siv hauv chav kuaj, nrog rau cov iav raj.
Ib qho gel clot endotoxin test assay yog siv los kuaj pom muaj cov endotoxins hauv cov qauv.Hauv qhov kev ntsuam xyuas no, ib qho txhaws tau tsim nyob rau hauv lub xub ntiag ntawm endotoxins.Qhov kev tsim cov qog no yog muab piv rau kev tswj hwm los txiav txim siab endotoxin concentration.
Siv cov iav tsis muaj endotoxin pab kom paub tseeb tias qhov kev kuaj pom endotoxin yog qhov tseeb.Qhov no yog vim hais tias endotoxins tuaj yeem ua raws li qhov chaw ntawm cov iav raj thiab cuam tshuam nrog cov txiaj ntsig kev soj ntsuam.
Txhawm rau kom ntseeg tau tias cov iav raj siv nyob rau hauv gel clot endotoxin test assay yog endotoxin-dawb, lawv yuav tsum tau ntxuav nrog xab npum thiab ces yaug nrog dej tsis muaj endotoxin.Tsis tas li ntawd, lawv yuav tsum tau sterilized siv autoclaving los yog qhuav cua sov kom tsis muaj menyuam.
Hauv kev xaus, kev siv cov iav tsis muaj endotoxin hauv cov gel clot endotoxin test assay yog qhov tseem ceeb los xyuas kom meej qhov tseeb ntawm endotoxins.Cov hlab no yuav tsum tau ntxuav kom huv si thiab ua kom tsis muaj menyuam kom tshem tawm tej yam kab mob.
Post lub sij hawm: Jun-02-2023
