Daim ntawv thov ntawm Chromogenic Technique rau Bacterial Endotoxins Test

Cov txheej txheem chromogenic yog ntawm peb cov txheej txheem uas tseem muaj cov txheej txheem gel-clot thiab cov txheej txheem turbidimetric los kuaj lossis ntsuas cov endotoxins los ntawm cov kab mob Gram-tsis zoo los ntawm kev siv amoebocyte lysate rho tawm los ntawm cov ntshav xiav ntawm horseshoe crab (Limulus polyphemus lossis Tachypleus tridentatus).Nws tuaj yeem raug cais raws li qhov kawg-chromogenic assay lossis kinetic-chromogenic assay raws li cov kev ntsuas tshwj xeeb ua haujlwm.

Lub hauv paus ntsiab lus ntawm cov tshuaj tiv thaiv yog tias: amebocyte lysate muaj ib qho cascade ntawm serine protease enzymes (proenzymes) uas tuaj yeem ua rau cov kab mob endotoxins.Endotoxins qhib cov proenzymes los tsim cov activated enzymes (lub npe coagulase), tom kawg catalyzes qhov sib cais ntawm cov xim tsis muaj xim, tso cov khoom daj xim pNA.Cov pNA tso tawm tuaj yeem ntsuas photometrically ntawm 405nm.Thiab qhov absorbance zoo sib raug zoo nrog cov endotoxin concentration, ces cov endotoxin concentration tuaj yeem raug xam raws li.