Endpoint Chromogenic cov khoom siv EC80545
Bioendo EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling)
1. Cov ntaub ntawv khoom
Bioendo EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling) muab kev ntsuas ceev rau endotoxin kom muaj nuj nqis.Cov endotoxin nyob rau hauv cov qauv activates ib tug cascade ntawm enzymes nyob rau hauv Amebocyte Lysate, lub activated enzyme splits cov hluavtaws substrate, tso cov khoom daj-xim.Tom qab ntawd cov khoom daj tuaj yeem cuam tshuam nrog diazo reagents los tsim cov khoom ntshav nrog qhov siab tshaj plaws absorbance ntawm 545nm.Yuav tsum muaj ib tus spectrophotometer tsis tu ncua lossis tus nyeem ntawv microplate rau qhov kev xeem.Cov khoom ntshav yog proportional rau endotoxin concentration.Tom qab ntawd cov txiaj ntsig ntawm kev ntsuas endotoxin yog qhov ntsuas ntau.
2. Khoom Parameter
rhiab heev: 0.01-0.1EU / ml (lub sij hawm soj ntsuam txog 46 feeb)
0.1-1.0EU / ml (lub sij hawm soj ntsuam txog 16 feeb)
3. Khoom nta thiab daim ntawv thov
Bioendo EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling) yog tsim los siv rau hauv Vitro nrhiav thiab kom muaj nuj nqis ntawm gram-negative bacterial endotoxins.Cov tshuaj tsis muaj xim tsis muaj xim peptide substrate yog ntxiv rau hauv Lyophilized Amebocyte Lysate, thiab tom qab ntawd kuaj cov qauv sib xyaw.Yog hais tias cov qauv muaj endotoxin, xim ntawm cov qauv sib tov hloov.Lub absorbance yog txuam nrog endotoxin concentration.Yog li cov qib endotoxn hauv cov qauv sib xyaw tuaj yeem raug claculated tiv thaiv tus qauv nkhaus.Standard spectrophotometer nrog 540 - 545nm lim yog txaus kom muaj nuj nqis endotoxin nrog peb EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling).
Nco tseg:
Lyophilized Amebocyte Lysate (LAL) reagent tsim los ntawm Bioendo yog tsim los ntawm amebocyte lysate derived ntshav ntawm horseshoe crab.
Catalog No. | Kev piav qhia | Cov khoom siv | Sensitivity EU/ml |
TIAB SA 80545 | Bioendo™ EC Endotoxin Test Kit (Qhov kawg-point Chromogenic Assay, Diazo Coupling), 80 Test/Kit | 5 Lyophilized Amebocyte Lysate, 1.7ml / vial; 4Dej rau BET, 50ml / vial; 5 CSE; 5 Chromogenic Substrate, 1.7ml / vial; 5 Diazo Reagent 1, 10ml / vial; 5 Diazo Reagent 2, 10ml / vial; 5 Diazo Reagent 3, 10ml / vial; | 0.1-1 EU / ml |
EC80545 S | 0.01 - 0.1 EU / ml; 0.1-1 EU / ml |
Lub rhiab heev ntawm Lyophilized Amebocyte Lysate thiab potency ntawm Control Standard Endotoxin raug soj ntsuam tawm tsam USP Reference Standard Endotoxin.Cov khoom siv Lyophilized Amebocyte Lysate reagent tuaj nrog cov khoom qhia, Daim Ntawv Pov Thawj Kev Ntsuam Xyuas.
Puas yog qhov kawg taw tes endotoxin kuaj cov khoom xav tau kev nyeem ntawv microplate?
Bioendo EC80545 thiab EC80545S, tuaj yeem nyeem los ntawm spectrophotometer li niaj zaus.